1A6F
RNASE P PROTEIN FROM BACILLUS SUBTILIS
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 64 |
| Unit cell lengths | 82.400, 82.400, 33.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.31700 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 25.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLOMON |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.086 * | 0.357 * |
| Total number of observations | 15148 * | |
| Number of reflections | 4058 | |
| <I/σ(I)> | 6 | 2.2 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | drop contains equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 24 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | mPEG550 | 23 (%) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | reservoir | MES | 100 (mM) |
