1A6E

THERMOSOME-MG-ADP-ALF3 COMPLEX

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1997-12-15
Detector	MARRESEARCH
Spacegroup name	I 4 2 2
Unit cell lengths	167.800, 167.800, 202.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.200
R-factor	0.181
Rwork	0.181
R-free	0.28500
Structure solution method	DIFFERENCE FOURIER SYNTHESIS
RMSD bond length	0.012
RMSD bond angle	21.700 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.280
High resolution limit [Å]	3.200	3.200
Rmerge	0.113 *	0.458 *
Number of reflections	22043
<I/σ(I)>	6.2	1.6
Completeness [%]	92.0	61.4
Redundancy	3.5	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.6	20 *	0.005ml of drop solution was mixed with 0.0025 ml of precipitant *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3.3 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		0.02 (%)
4	1	reservoir	ammonium sulfate	2 (M)	precipitant
5	1	reservoir	sodium acetate	0.1 (M)	precipitant