1A6D
THERMOSOME FROM T. ACIDOPHILUM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 168.300, 168.300, 203.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.29800 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 22.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | SHELX-96 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.800 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.066 | 0.289 |
| Number of reflections | 42095 | |
| <I/σ(I)> | 8.6 | 2.5 |
| Completeness [%] | 93.6 | 77.3 |
| Redundancy | 3.9 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.6 | 20 * | 0.005ml of drop solution was mixed with 0.0025 ml of precipitant * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 0.02 (%) | ||
| 4 | 1 | reservoir | ammonium sulfate | 2 (M) | precipitant |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) | precipitant |
