1A68

CRYSTAL STRUCTURE OF THE TETRAMERIZATION DOMAIN OF THE SHAKER POTASSIUM CHANNEL

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	290
Detector technology	CCD AREA DETECTOR
Collection date	1995-08
Detector	SIEMENS
Spacegroup name	I 4
Unit cell lengths	51.034, 51.034, 65.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.2
Rwork	0.200
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	24.400 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.063 *	0.035 *
Number of reflections	7712
<I/σ(I)>	8.8	3.5
Completeness [%]	99.0	97.2
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5	15 *	PROTEIN WAS CRYSTALLIZED FROM 7-8% PEG8000, 300 MM NACL, 200MM MGCL2, 20 MM TRIS, PH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	7-8 (%)
2	1	1		300 (mM)
3	1	1		200 (mM)
4	1	1	Tris-HCl	20 (mM)