1A65

TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	273
Detector technology	IMAGE PLATE
Collection date	1996-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	45.390, 85.720, 143.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 2.230
R-factor	0.16 *
Rwork	0.160
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1aoz
RMSD bond length	0.011
RMSD bond angle	0.033
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.350
High resolution limit [Å]	2.200	2.230
Rmerge	0.100	0.270
Number of reflections	152295
<I/σ(I)>	8.4	4.8
Completeness [%]	99.6	98.5
Redundancy	5.4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5	291 *	Ducros, V., (1997) Acta Crystallogr.,Sect.D, D53, 605. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	acetate	10 (mM)
3	1	reservoir	PEG8000	26-30 (%(v/v))
4	1	reservoir	acetate	0.1 (M)