1A64
ENGINEERING A MISFOLDED FORM OF RAT CD2
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 40.720, 40.720, 105.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.2306 |
| Rwork | 0.231 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hng |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.623 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.090 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.043 | 0.271 |
| Number of reflections | 11660 | |
| <I/σ(I)> | 19.87 | 3.15 |
| Completeness [%] | 97.8 | 99.8 |
| Redundancy | 6.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 20% MPD, 200MM NACL, 0.1M NACO, PH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | MPD | 20 (%) | |
| 2 | 1 | reservoir | 200 (mM) | ||
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) |
