1A62
CRYSTAL STRUCTURE OF THE RNA-BINDING DOMAIN OF THE TRANSCRIPTIONAL TERMINATOR PROTEIN RHO
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05 |
| Detector | FUJI |
| Wavelength(s) | 0.969, 0.978, 0.979, 0.984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.950, 56.010, 42.920 |
| Unit cell angles | 90.00, 99.35, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.25000 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.320 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.068 | 0.270 |
| Number of reflections | 16360 | |
| <I/σ(I)> | 22 | 5.2 |
| Completeness [%] | 92.0 | 67 |
| Redundancy | 6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 1 (mM) | |
| 2 | 1 | 1 | potassium sulfate | 200 (mM) |
