1A5Z

LACTATE DEHYDROGENASE FROM THERMOTOGA MARITIMA (TMLDH)

Experimental procedure

Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-07
Detector	MARRESEARCH
Spacegroup name	I 41 2 2
Unit cell lengths	105.480, 105.480, 187.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.100
R-factor	0.2
Rwork	0.200
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.910
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.100
Rmerge	0.089
Total number of observations	111661 *
Number of reflections	30205
Completeness [%]	97.9 *	96.5 *
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.0 (mg/ml)
2	1	drop	HEPES	0.1 (M)
3	1	reservoir	sodium acetate	1.0 (M)
4	1	reservoir	oxamate	250 (mM)
5	1	reservoir	NADH	150 (mM)
6	1	reservoir	fructose-1,6-bisphosphate	150 (mM)
7	1	reservoir	cadmium sulfate	50 (mM)