1A5X
ASV INTEGRASE CORE DOMAIN WITH HIV-1 INTEGRASE INHIBITOR Y3
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-17 |
| Detector | MACSCIENCE |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 66.443, 66.443, 81.282 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.155 |
| Rwork | 0.155 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1asv |
| RMSD bond length | 0.012 |
| RMSD bond angle | 23.370 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.058 * | 0.373 * |
| Total number of observations | 115637 * | |
| Number of reflections | 14942 | |
| <I/σ(I)> | 16.6 | |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 20% PEG 4000, 10% ISOPROPANOL, 0.1M HEPES, PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6-7.5 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | |
| 3 | 1 | reservoir | isopropanol | 10 (%) | |
| 4 | 1 | reservoir | PEG4000 | ||
| 5 | 1 | drop | inhibitor | 0.1 (M) |
