1A5U
PYRUVATE KINASE COMPLEX WITH BIS MG-ATP-NA-OXALATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 113 |
| Collection date | 1996-05 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.300, 216.500, 258.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.19 * |
| Rwork | 0.190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aqf |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.100 |
| Phasing software | AMoRE |
| Refinement software | TNT (5D) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.460 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.053 * | 0.164 * |
| Total number of observations | 717630 * | |
| Number of reflections | 193413 | |
| Completeness [%] | 90.0 | 74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6 | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 7.1 (mg/ml) | |
| 2 | 1 | 1 | PEG8000 | 7.8 (%) | |
| 3 | 1 | 1 | 0.16 (M) | ||
| 4 | 1 | 1 | 5 (mM) | ||
| 5 | 1 | 1 | ATP | 5 (mM) | |
| 6 | 1 | 1 | oxalate | 1 (mM) | |
| 7 | 1 | 1 | succinic | 50 (mM) |
