1A5T

CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III

Experimental procedure

Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1994-07
Detector	RIGAKU
Spacegroup name	C 2 2 21
Unit cell lengths	98.900, 104.000, 75.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.205
Rwork	0.205
R-free	0.26500
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	2.150
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.074 *	0.353 *
Number of reflections	19389
<I/σ(I)>	23	6
Completeness [%]	96.0	93
Redundancy	5.3	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8	4 *	THE PROTEIN WAS CRYSTALLIZED FROM 20-27% PEG 400, 100 MM HEPES, PH6.8, 100 MM MGCL2, 1-3% GLYCEROL, 10MM MGSO4, AT 4C, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	PEG400	20-27 (%)
3	1	reservoir	HEPES	100 (mM)
4	1	reservoir		100 (mM)
5	1	reservoir	glycerol	1-3 (%)
6	1	reservoir		10 (mM)