1A5T
CRYSTAL STRUCTURE OF THE DELTA PRIME SUBUNIT OF THE CLAMP-LOADER COMPLEX OF ESCHERICHIA COLI DNA POLYMERASE III
Experimental procedure
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-07 |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 98.900, 104.000, 75.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.26500 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.150 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.074 * | 0.353 * |
| Number of reflections | 19389 | |
| <I/σ(I)> | 23 | 6 |
| Completeness [%] | 96.0 | 93 |
| Redundancy | 5.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.8 | 4 * | THE PROTEIN WAS CRYSTALLIZED FROM 20-27% PEG 400, 100 MM HEPES, PH6.8, 100 MM MGCL2, 1-3% GLYCEROL, 10MM MGSO4, AT 4C, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | PEG400 | 20-27 (%) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | glycerol | 1-3 (%) | |
| 6 | 1 | reservoir | 10 (mM) |
