1A59
COLD-ACTIVE CITRATE SYNTHASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 70.800, 70.800, 307.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.090 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.240 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.160 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.050 | 0.120 |
| Total number of observations | 293261 * | |
| Number of reflections | 27063 | |
| <I/σ(I)> | 23.9 | 7.7 |
| Completeness [%] | 96.2 | 88.3 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 29 * | pH 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | CoASH | 10 (mM) | |
| 5 | 1 | drop | citrate | 10 (mM) | |
| 6 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
| 7 | 1 | reservoir | citrate | 20 (mM) |
