1A58

CYCLOPHILIN FROM BRUGIA MALAYI

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	58.215, 58.215, 141.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.000 - 1.950
R-factor	0.199
Rwork	0.199
R-free	0.23400 *
RMSD bond length	0.008
RMSD bond angle	26.020 *
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
High resolution limit [Å]	1.950 *
Rmerge	0.092 *
Total number of observations	103228 *
Number of reflections	18055 *
Completeness [%]	96.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		50MM IMIDAZOLE AT PH6.5, 15% SAT. AMMONIUM SULFATE 0.02% SODIUM AZIDE, 0.56 MM PROTEIN CONC.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	imidazole	50 (mM)
2	1	drop	ammonium sulfate	15 (%sat)
3	1	drop		0.02 (%(w/v))
4	1	drop	bmCyp-1	0.56 (mM)
5	1	reservoir	imidazole	100 (mM)
6	1	reservoir	ammonium sulfate	30 (%sat)
7	1	reservoir		0.02 (%(w/v))