1A53

COMPLEX OF INDOLE-3-GLYCEROLPHOSPHATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS WITH INDOLE-3-GLYCEROLPHOSPHATE AT 2.0 A RESOLUTION

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1994-08-10
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	57.900, 73.800, 104.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.000
R-factor	0.159
Rwork	0.159
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1igs
RMSD bond length	0.012
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.063	0.390
Total number of observations	141730 *
Number of reflections	28014 *
<I/σ(I)>	10	1.6
Completeness [%]	91.1	48
Redundancy	5.1	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5	4 *	pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	20 (mg/ml)
2	1	drop	ammonium sulfate	26-30 (%sat)
3	1	reservoir	ammonium sulfate	26-30 (%sat)