1A50
CRYSTAL STRUCTURE OF WILD-TYPE TRYPTOPHAN SYNTHASE COMPLEXED WITH 5-FLUOROINDOLE PROPANOL PHOSPHATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1995-11 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.700, 60.700, 67.500 |
| Unit cell angles | 90.00, 94.50, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.164 * |
| Rwork | 0.177 |
| R-free | 0.22100 * |
| Structure solution method | ISOMORPHOUS WITH PDB ENTRY 1WSY. |
| Starting model (for MR) | 1WSY |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.900 | 2.340 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.060 | 0.210 |
| Total number of observations | 66781 * | |
| Number of reflections | 31823 | |
| <I/σ(I)> | 13.4 | 6.4 |
| Completeness [%] | 95.6 | 91.6 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.8 | pH 7.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 10 (mg/ml) | |
| 2 | 1 | drop | Bicine | 50 (mM) | |
| 3 | 1 | drop | pyridoxal phosphate | 0.02 (M) | |
| 4 | 1 | reservoir | bicine | 50 (mM) | |
| 5 | 1 | reservoir | spermine | 1-2 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 10 (%) |
