1A4P
P11 (S100A10), LIGAND OF ANNEXIN II
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE D41A |
| Synchrotron site | LURE |
| Beamline | D41A |
| Temperature [K] | 280 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-18 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 57.750, 80.750, 95.510 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.227 * |
| Rwork | 0.227 |
| R-free | 0.30300 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.037 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.032 * | 0.168 * |
| Total number of observations | 67368 * | |
| Number of reflections | 10957 * | |
| <I/σ(I)> | 14.9 | 3.5 |
| Completeness [%] | 98.4 | 97.1 |
| Redundancy | 6.15 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | drop:reservoir=1:1 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Na acetate | 50 (mM) | |
| 2 | 1 | drop | 250 (mM) | ||
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 20 (%) | |
| 6 | 1 | reservoir | 2-propanol | 10 (%) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) |
