1A4G

INFLUENZA VIRUS B/BEIJING/1/87 NEURAMINIDASE COMPLEXED WITH ZANAMIVIR

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-02
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	88.200, 88.200, 221.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.000 - 2.200
R-factor	0.198
Rwork	0.198
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	1nsc
RMSD bond length	0.009
RMSD bond angle	27.800 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.000
High resolution limit [Å]	2.200
Rmerge	0.128
Total number of observations	140043 *
Number of reflections	50283 *
Completeness [%]	98.0
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	seeding *	7.8		pH 7.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000	7-10 (%)
2	1	1	Tris-HCl	100 (mM)