1A4F
BAR-HEADED GOOSE HEMOGLOBIN (OXY FORM)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY |
| Synchrotron site | Photon Factory |
| Temperature [K] | 283 |
| Detector technology | FILM |
| Collection date | 1992-07 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 81.590, 81.590, 107.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.052 |
| Data reduction software | WEIS |
| Phasing software | AUTOMR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.051 |
| Total number of observations | 92268 * |
| Number of reflections | 24887 |
| Completeness [%] | 87.2 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG6000 | 10 (%) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | drop | protein | 25 (mg/ml) | |
| 4 | 1 | reservoir | EDTA | 1 (mM) |
