1A3Y

ODORANT BINDING PROTEIN FROM NASAL MUCOSA OF PIG

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-06
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	42.360, 88.730, 93.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.250
R-factor	0.181
Rwork	0.181
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1obp
RMSD bond length	0.008
RMSD bond angle	26.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.200
High resolution limit [Å]	2.150	2.150
Rmerge	0.051 *	0.265 *
Number of reflections	11000
<I/σ(I)>	12	2
Completeness [%]	99.4	99
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.5	20 *	pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	2-propanol	5 (%)