Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

1A3V

STAPHYLOCOCCAL NUCLEASE, CYCLOPENTANE THIOL DISULFIDE TO V23C VARIANT

Experimental procedure
Source typeROTATING ANODE
Source detailsRIGAKU RUH2R
Temperature [K]278.15
Detector technologyIMAGE PLATE
Collection date1996-01
DetectorMACSCIENCE
Spacegroup nameP 41
Unit cell lengths48.200, 48.200, 63.200
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution6.000 - 2.200
R-factor0.174
Rwork0.174
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1snc
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareX-PLOR (3.1)
Refinement softwareX-PLOR (3.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]6.0002.300
High resolution limit [Å]2.2002.200
Rmerge0.062
Number of reflections5789

*

Completeness [%]82.574.3
Redundancy5.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1Vapor diffusion

*

8.154

*

Loll, P.J., (1989) Proteins Struct. Funct. Genet., 5, 183.

*

Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropprotein20 (mg/ml)
21droppotassium phosphate10.5 (mM)
31dropMPD17 (%(w/w))
41drop10 (mM)
51droppotassium citrate20 (mM)
61reservoirpotassium phosphate10.5 (mM)
71reservoirMPD21-23 (%(w/w))

224201

PDB entries from 2024-08-28

PDB statisticsPDBj update infoContact PDBjnumon