1A3N

DEOXY HUMAN HEMOGLOBIN

Experimental procedure

Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1997-05
Detector	RIGAKU RAXIS II
Spacegroup name	P 1 21 1
Unit cell lengths	62.650, 82.430, 53.530
Unit cell angles	90.00, 99.61, 90.00

Refinement procedure

Resolution	15.000 - 1.800
R-factor	0.171 *
Rwork	0.171
R-free	0.22038
RMSD bond length	0.012
RMSD bond angle	0.028
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.053	0.162
Number of reflections	49661
<I/σ(I)>	15	4
Completeness [%]	95.8	76.1
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5 *		Perutz, M.F., (1968) J. Cryst. Growth, 2, 54. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	4 (M)
2	1	1		2 (M)
3	1	1		2 (M)