1A3C
PYRR, THE BACILLUS SUBTILIS PYRIMIDINE BIOSYNTHETIC OPERON REPRESSOR, DIMERIC FORM
Experimental procedure
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-09 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.700, 57.700, 55.000 |
Unit cell angles | 90.00, 129.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.600 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.23300 |
Structure solution method | MAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.580 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 1.550 |
Rmerge | 0.085 * |
Total number of observations | 104445 * |
Number of reflections | 26609 |
<I/σ(I)> | 11.7 |
Completeness [%] | 98.0 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * | PROTEIN WAS CRYSTALLIZED FROM 13-16% PEG 6000, 300 MM AMMONIUM SULFATE, 0.2% BETA-OCTYLGLUCOSIDE, 50 MM SODIUM SUCCINATE, PH 5.1, AND 1 MM SAMARIUM NITRATE. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-acetate | 10 (mM) | pH7.5 |
3 | 1 | drop | beta-octylglucoside | 0.2 (%) | |
4 | 1 | reservoir | PEG6000 | 13-16 (%) | |
5 | 1 | reservoir | ammonium sulfate | 300 (mM) | |
6 | 1 | reservoir | sodium succinate | 50 (mM) | pH5.1 |