1A39

HUMICOLA INSOLENS ENDOCELLULASE EGI S37W, P39W DOUBLE-MUTANT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1996-07
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	50.960, 81.400, 94.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
Rwork	0.170
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NATIVE HUMICOLA INSOLENS ENDOGLUCANASE I
RMSD bond length	0.011
RMSD bond angle	0.034
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.067	0.200
Number of reflections	18592
<I/σ(I)>	14	4
Completeness [%]	91.0	51
Redundancy	3.4	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5		pH 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	mPEG2000
2	1	reservoir	Tris-HCl	50 (mM)