1A33
PEPTIDYLPROLYL ISOMERASE, CYCLOPHILIN-LIKE DOMAIN FROM BRUGIA MALAYI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-07 |
| Detector | MACSCIENCE |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.900, 57.900, 140.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.150 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cwa |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.750 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.050 | 0.080 |
| Total number of observations | 84704 * | |
| Number of reflections | 13361 | |
| <I/σ(I)> | 36 | 22 |
| Completeness [%] | 98.6 | 98.6 * |
| Redundancy | 6.4 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 19 * | THE INITIAL 6 UL DROP CONSISTED OF 50 MM MES-NAOH PH 6.0, 0.9 M (NH4)2SO4, 1MM PROTEIN. THE 1 ML RESERVOIR SOLUTION CONSISTED OF 100 MM MES-NAOH PH 6.0, 1.8 M (NH4)2SO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | reservoir | MES-NaOH | 100 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.8 (M) | |
| 4 | 1 | drop | MES-NaOH | 50 (mM) | |
| 5 | 1 | drop | ammonium sulfate | 0.9 (M) |
