1A32

RIBOSOMAL PROTEIN S15 FROM BACILLUS STEAROTHERMOPHILUS

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-05
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	33.020, 33.750, 75.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.100
R-factor	0.212
Rwork	0.212
R-free	0.31800
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.140
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.130
High resolution limit [Å]	2.100	2.050
Rmerge	0.075	0.343 *
Number of reflections	139136
<I/σ(I)>	12	3
Completeness [%]	99.5	99.2
Redundancy	4.5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	23 *	3.0M NA/K PHOSPHATE PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Na/KPO4	2.4-3.0 (M)