1A2Y

HEN EGG WHITE LYSOZYME, D18A MUTANT, IN COMPLEX WITH MOUSE MONOCLONAL ANTIBODY D1.3

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS
Synchrotron site	CHESS
Temperature [K]	100
Detector technology	CCD
Collection date	1997-07
Spacegroup name	C 1 2 1
Unit cell lengths	130.667, 61.212, 57.425
Unit cell angles	90.00, 119.14, 90.00

Refinement procedure

Resolution	7.000 - 1.500
R-factor	0.203
Rwork	0.203
R-free	0.25100
Starting model (for MR)	1vfb
RMSD bond length	0.012
RMSD bond angle	25.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.069	0.301
Total number of observations	403840 *
Number of reflections	59595
Completeness [%]	90.4	50.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	16 (%(w/v))
2	1	reservoir	potassium phosphate	0.1 (M)