1A2X
COMPLEX OF TROPONIN C WITH A 47 RESIDUE (1-47) FRAGMENT OF TROPONIN I
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 46.900, 46.900, 152.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.32500 |
| Structure solution method | SIR/MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.050 | 0.300 |
| Total number of observations | 37214 * | |
| Number of reflections | 8870 | |
| <I/σ(I)> | 9.2 | 2.3 |
| Completeness [%] | 96.3 | 94 |
| Redundancy | 4.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 16 * | Saijo, Y., (1997) Protein Sci., 6, 916. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25-30 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 1.5 (M) | |
| 3 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.0 |
| 4 | 1 | reservoir | trehalose | 15 (%) |
