1A2W
CRYSTAL STRUCTURE OF A 3D DOMAIN-SWAPPED DIMER OF BOVINE PANCREATIC RIBONUCLEASE A
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10 |
Detector | RIGAKU |
Spacegroup name | P 32 |
Unit cell lengths | 57.000, 57.000, 81.410 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.192 |
Rwork | 0.192 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rtb |
RMSD bond length | 0.010 |
RMSD bond angle | 25.800 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.097 * | |
Total number of observations | 30428 * | |
Number of reflections | 16601 | |
<I/σ(I)> | 8 | 2 |
Completeness [%] | 96.0 | 92 |
Redundancy | 1.8 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | used to seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | dimer | 7.5 (mg/ml) | |
10 | 1 | reservoir | 0.6 (M) | ||
11 | 1 | reservoir | PEG400 | 2 (%) | |
2 | 1 | drop | phosphate | 55 (mM) | |
3 | 1 | drop | sodium sulfate | 0.05 (M) | |
4 | 1 | drop | ADP | 5 (mM) | |
5 | 1 | drop | ammonium sulfate | 0.8 (M) | |
6 | 1 | drop | 0.3 (M) | ||
7 | 1 | drop | PEG400 | 1 (%) | |
8 | 1 | reservoir | phosphate | 100 (mM) | |
9 | 1 | reservoir | ammonium sulfate | 1.6 (M) |