1A2V

COPPER AMINE OXIDASE FROM HANSENULA POLYMORPHA

Experimental procedure

Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-12
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	138.770, 148.220, 234.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 2.400
R-factor	0.184
Rwork	0.184
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT AND SIR
Starting model (for MR)	1oac
RMSD bond length	0.011
RMSD bond angle	27.900 *
Data reduction software	DENZO
Data scaling software	SCALEIT
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.550
High resolution limit [Å]	2.400	2.400
Rmerge	0.053	0.247
Number of reflections	172832
<I/σ(I)>	12.5	2.4
Completeness [%]	87.2	66.8
Redundancy	2.8	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *		PROTEIN WAS CRYSTALLIZED IN SITTING DROPS FROM 7-9% PEG 8000 AND 0.3 M POTASSIUM PHOSPHATE BUFFER, PH 6.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	potassium phosphate	155 (mM)
3	1	drop	PEG8000	3.5-4.5 (%)
4	1	reservoir	potassium phosphate	0.3 (M)
5	1	reservoir	PEG8000	7-9 (%)