1A2Q
SUBTILISIN BPN' MUTANT 7186
Experimental procedure
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-21 |
Temperature [K] | 290 |
Detector technology | AREA DETECTOR |
Collection date | 1987-01-31 |
Detector | SIEMENS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.640, 79.450, 37.260 |
Unit cell angles | 90.00, 114.53, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.800 |
R-factor | 0.145 * |
Rwork | 0.145 |
Structure solution method | DIFFERENCE MAP |
RMSD bond length | 0.019 |
RMSD bond angle | 0.030 |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Refinement software | PROFFT |
Data quality characteristics
Overall | |
High resolution limit [Å] | 1.750 |
Number of reflections | 14470 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 9 * | Bryan, P.N., (1986) Proteins: Struct.,Funct., Genet., 1, 326. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | acetone | 55 (%) | |
2 | 1 | drop | glycine-NaOH | 50 (mM) | pH9.0 |