1A2P

BARNASE WILDTYPE STRUCTURE AT 1.5 ANGSTROMS RESOLUTION

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1993-11
Detector	MARRESEARCH
Spacegroup name	P 32
Unit cell lengths	58.970, 58.970, 81.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 1.500
R-factor	0.111
R-free	0.17400
RMSD bond length	0.013
RMSD bond angle	1.760
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	SHELXL-93
Refinement software	SHELXL-93

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.038 *
Number of reflections	48463
<I/σ(I)>	11.9	4.7
Completeness [%]	96.9	94.5
Redundancy	2.5	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5 (mg/ml)
2	1	drop	ammonium sulfate	3.0 (M)
3	1	drop	zinc sulfate	0.03 (M)
4	1	reservoir	phosphate	1.9 (M)