1A2B
HUMAN RHOA COMPLEXED WITH GTP ANALOGUE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.020, 74.780, 50.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5p21 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.360 * |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.500 | |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 0.267 |
| Total number of observations | 61579 * | |
| Number of reflections | 8683 | |
| <I/σ(I)> | 7.91 | 2.04 |
| Completeness [%] | 89.3 | 74.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 4 * | CRYSTALS WERE OBTAINED AT 277 K BY THE HANGING-DROP VAPOR DIFFUSION METHOD FROM SOLUTIONS CONTAINING 10 MG/ML(PROTEIN,GTPGAMMAS,MG2+ MIXTURE), 10% PEG 8000,7.5% 14-DIOXANE, 50 MM TRIS-HCL PH 8.5, EQUILIBRATED AGAINST 20% PEG 8000,15% 14-DIOXANE, 100 MM TRIS-HCL PH 8.5, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris/HCl | 50 (mM) | |
| 3 | 1 | drop | PEG8000 | 10 (%) | |
| 4 | 1 | drop | 1,4-dioxane | 7.5 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 20 (%) | |
| 7 | 1 | reservoir | 1,4-dioxane | 15 (%) |
