1A21
TISSUE FACTOR (TF) FROM RABBIT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-03 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.540, 49.010, 65.010 |
| Unit cell angles | 96.06, 99.11, 91.14 |
Refinement procedure
| Resolution | 10.000 - 2.350 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hft |
| RMSD bond length | 0.012 |
| RMSD bond angle | 26.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.400 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.075 | 0.070 * |
| Number of reflections | 20678 | |
| Completeness [%] | 96.4 | 90.6 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | SITTING DROP, 80 UL OF PROTEIN SOLUTION (10 MG/ML PROTEIN IN 50 MM TRIS*HCL,PH:8.0, 50 MM NACL, 75 MM LI2SO4, 12.5 % PEG 3350) EQUILIBRATED AGAINST 30 ML RESERVOIR SOLUTION (50 MM TRIS*HCL, PH:8.0, 50 MM NACL, 150 MM LI2SO4, 25 % PEG3350), vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | 75 (mM) | ||
| 5 | 1 | drop | PEG3350 | 12.5 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 7 | 1 | reservoir | 50 (mM) | ||
| 8 | 1 | reservoir | 150 (mM) | ||
| 9 | 1 | reservoir | PEG3350 | 25 (%) |
