1A1X

CRYSTAL STRUCTURE OF MTCP-1 INVOLVED IN T CELL MALIGNANCIES

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	RIGAKU
Spacegroup name	P 62 2 2
Unit cell lengths	62.665, 62.665, 85.962
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.500 - 2.000
R-factor	0.211
Rwork	0.211
R-free	0.25300
Structure solution method	MAD PHASING
RMSD bond length	0.007
RMSD bond angle	26.920 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MADSYS
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.047 *	0.147 *
Number of reflections	7194
<I/σ(I)>	15.1	7.6
Completeness [%]	99.7	99.3
Redundancy	5.9	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8		PROTEIN WAS CRYSTALLIZED FROM 1.5M AMS WITH TRIZMA BUFFER AT PH 7.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5.0 (mg/ml)
2	1	reservoir	ammonium sulfate	1.5 (M)
3	1	reservoir	2-mercaptoethanol	1.0 (mM)