1A1C
C-SRC (SH2 DOMAIN) COMPLEXED WITH ACE-PHOSPHOTYR-GLU-(N-ME(-(CH2)3-CYCLOPENTYL))
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.700, 66.900, 75.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.400 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shd |
| RMSD bond length | 0.017 |
| RMSD bond angle | 23.241 * |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.061 | 0.178 |
| Number of reflections | 9865 | |
| <I/σ(I)> | 11 | 3.4 |
| Completeness [%] | 91.0 | 83 |
| Redundancy | 3.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 22 * | PROTEIN WAS CRYSTALLIZED FROM 0.1 M ACETATE, PH 4.6,2M AMMONIUM SULFATE AT 22 C., temperature 295K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 125 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 350 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | EDTA | 5 (mM) | |
| 6 | 1 | reservoir | acetate | 0.1 (M) | |
| 7 | 1 | reservoir | ammonium sulfate | 2 (M) |
