1A19
BARSTAR (FREE), C82A MUTANT
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-12 |
Detector | MARRESEARCH |
Spacegroup name | I 41 |
Unit cell lengths | 104.182, 104.182, 36.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.760 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.29200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1BGS 1brs |
RMSD bond length | 0.008 |
RMSD bond angle | 26.800 * |
Data reduction software | MARXDS |
Data scaling software | MARSCALE |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.900 |
High resolution limit [Å] | 2.760 | 2.800 |
Rmerge | 0.076 | 0.050 |
Number of reflections | 4796 | |
<I/σ(I)> | 33 | |
Completeness [%] | 99.0 * | 50 |
Redundancy | 7 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 | 20 * | C82A MUTANT WAS CRYSTALLIZED FROM 40-60% AMMONIUM SULFATE, 50MM PHOSPHATE, PH=6.5. PROTEIN CONC=45 MG/ML. C40A AND DTNB LABELLED CRYSTALS ALSO CRYSTALLIZED BUT DID NOT DIFFRACT TO HIGH RESOLUTION. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 22.5 (mg/ml) | |
2 | 1 | drop | phosphate | 50 (mM) | |
3 | 1 | drop | ammonium sulfate | 20-30 (%) |