1A17
TETRATRICOPEPTIDE REPEATS OF PROTEIN PHOSPHATASE 5
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 90.060, 90.060, 104.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.450 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.29800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.012 * |
| RMSD bond angle | 1.754 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | X-PLOR (3.82) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.073 | 0.133 |
| Number of reflections | 7832 | |
| <I/σ(I)> | 24.4 | 3 |
| Completeness [%] | 96.8 | 97.6 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 4 * | 1.8 M AMMONIUM SULFATE, 4% MPD, 100 MM HEPES PH 7.5, 2 MM DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium salfate | 1.8 (M) | |
| 2 | 1 | reservoir | MPD | 4 (%(v/v)) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | |
| 4 | 1 | reservoir | dithiothreitol | 2 (mM) |
