1A16
AMINOPEPTIDASE P FROM E. COLI WITH THE INHIBITOR PRO-LEU
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 1996-08 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 177.300, 177.300, 96.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.184 |
Rwork | 0.184 |
R-free | 0.22600 |
Structure solution method | MIR |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.8) |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.300 | 2.300 * |
Rmerge | 0.096 * | 0.375 * |
Total number of observations | 549017 * | |
Number of reflections | 36900 * | |
<I/σ(I)> | 18 | 4.5 |
Completeness [%] | 91.9 * | 92 * |
Redundancy | 19.2 | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.3 | drop contained 1:1 mixture of protein and reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8.7 (mg/ml) | |
2 | 1 | drop | 5 (mM) | ||
3 | 1 | reservoir | PEG4000 | 30-33 (%) | |
4 | 1 | reservoir | Tris-HCl | 100 (mM) |