1A15
SDF-1ALPHA
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 133 |
Detector technology | IMAGE PLATE |
Collection date | 1997-09 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.840, 50.470, 64.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 5.000 - 2.200 |
R-factor | 0.233 |
Rwork | 0.233 |
R-free | 0.29800 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT WITH ANOMALOUS SCATTERING (MIRAS) |
RMSD bond length | 0.006 |
RMSD bond angle | 26.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.843) |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.046 | 0.087 |
Total number of observations | 22469 * | |
Number of reflections | 8725 | |
<I/σ(I)> | 9.3 | 3.1 |
Completeness [%] | 97.6 | 99 |
Redundancy | 2.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | PROTEIN CONCENTRATION 10MG/ML. WELL SOLUTION 1.9M AMMONIUM SULFATE 0.1M TRIS-HCL PH 8.5. CRYSTALLIZED USING VAPOR DIFFUSION., vapor diffusion |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 12 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 1.9 (M) | |
3 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |