1A15

SDF-1ALPHA

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	133
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	38.840, 50.470, 64.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.200
R-factor	0.233
Rwork	0.233
R-free	0.29800
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT WITH ANOMALOUS SCATTERING (MIRAS)
RMSD bond length	0.006
RMSD bond angle	26.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.046	0.087
Total number of observations	22469 *
Number of reflections	8725
<I/σ(I)>	9.3	3.1
Completeness [%]	97.6	99
Redundancy	2.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5		PROTEIN CONCENTRATION 10MG/ML. WELL SOLUTION 1.9M AMMONIUM SULFATE 0.1M TRIS-HCL PH 8.5. CRYSTALLIZED USING VAPOR DIFFUSION., vapor diffusion

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	reservoir	ammonium sulfate	1.9 (M)
3	1	reservoir	Tris	0.1 (M)	pH8.5