1A14
COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 5 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07 |
| Detector | PHOTON FACTORY |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 165.300, 165.300, 182.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 2.500 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nmc |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.900 |
| Data reduction software | PROTEIN |
| Data scaling software | PROTEIN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.600 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.118 | |
| Total number of observations | 86319 * | |
| Number of reflections | 29660 | |
| Completeness [%] | 68.0 | 40 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.6 | 1 was mixed with 2 in the molar ration 1:4, and an equal volume of 3 was added. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | neuraminidase | 10 (mg/ml) | 1 |
| 2 | 1 | drop | phosphate-beffered saline | 1 | |
| 3 | 1 | drop | scFv(5) | 5 (mg/ml) | 2 |
| 4 | 1 | drop | potassium phosphate | 1.7 (M) | 3 |
| 5 | 1 | reservoir | potassium phosphate | 1.3 (M) |
