1A14

COMPLEX BETWEEN NC10 ANTI-INFLUENZA VIRUS NEURAMINIDASE SINGLE CHAIN ANTIBODY WITH A 5 RESIDUE LINKER AND INFLUENZA VIRUS NEURAMINIDASE

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	1995-07
Detector	PHOTON FACTORY
Spacegroup name	I 4 2 2
Unit cell lengths	165.300, 165.300, 182.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.500
R-factor	0.2
Rwork	0.200
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nmc
RMSD bond length	0.014
RMSD bond angle	1.900
Data reduction software	PROTEIN
Data scaling software	PROTEIN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.118
Total number of observations	86319 *
Number of reflections	29660
Completeness [%]	68.0	40

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.6		1 was mixed with 2 in the molar ration 1:4, and an equal volume of 3 was added. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	neuraminidase	10 (mg/ml)	1
2	1	drop	phosphate-beffered saline		1
3	1	drop	scFv(5)	5 (mg/ml)	2
4	1	drop	potassium phosphate	1.7 (M)	3
5	1	reservoir	potassium phosphate	1.3 (M)