1A0S
SUCROSE-SPECIFIC PORIN
Experimental procedure
| Source details | OTHER |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 |
| Unit cell lengths | 112.100, 112.100, 147.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 100.000 - 2.400 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.22800 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 28.300 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | DAREFI |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 2.400 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.085 * | 0.301 * |
| Number of reflections | 130884 | |
| <I/σ(I)> | 8 | 3 |
| Completeness [%] | 92.6 | 65 |
| Redundancy | 1.58 | 1.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 17 * | PROTEIN WAS CRYSTALLIZED BY VAPOR DIFFUSION USING THE SITTING-DROP METHOD. THE DROP CONTAINED 5-7 MG/ML PROTEIN, 20 MM TRIS/CL AT PH 7.7, 100MM LICL, 20MM MGSO4, 1.2% BETA-D-OCTYLGLUCOPYRANOSIDE AND 6-9% PEG-2000. THE CONCENTRATION OF PEG IN THE RESERVOIR WAS 12-15%., vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-7 (mg/ml) | |
| 10 | 1 | drop | sucrose | 100 (mM) | |
| 2 | 1 | drop | Tris/Cl | 20 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | 20 (mM) | ||
| 5 | 1 | drop | beta-D-octylglucopyranoside | 1.2 (%) | |
| 6 | 1 | drop | Hexyldimethylamineoxide | 1 (%) | |
| 7 | 1 | drop | btea-D-heptylglucopyranoside | 1 (%) | |
| 8 | 1 | drop | PEG2000 | 6-9 (%) | |
| 9 | 1 | reservoir | PEG2000 | 12-15 (%) |
