1A0R
HETEROTRIMERIC COMPLEX OF PHOSDUCIN/TRANSDUCIN BETA-GAMMA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM02 |
| Synchrotron site | ESRF |
| Beamline | BM02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-04 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.090, 87.910, 98.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.222 * |
| Rwork | 0.228 |
| R-free | 0.26100 * |
| Structure solution method | MR, CROSS-CRYSTAL A |
| Starting model (for MR) | BETA-GAMMA CHAIN OF 1GP2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 23.800 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 * | 0.315 * |
| Number of reflections | 15784 | |
| Completeness [%] | 93.2 | 97.8 |
| Redundancy | 3.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 6.8 | 4 * | THE PROTEIN COMPLEX (10 MG/ML SOLUTION) WAS CRYSTALLIZED FROM 400 MM SODIUM CACODYLATE (PH 6.8), 1 MM ZINC CHLORIDE, 25 % ETHYLENE GLYCOL, 12 % PEG 8000, BY MICROBATCH CRYSTALLIZATION AT 4 DEGREES C., microbatch, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | cacodylic acid/NaOH | 400 (mM) | |
| 3 | 1 | 1 | 1 (mM) | ||
| 4 | 1 | 1 | beta-mercaptoethanol | 1 (mM) | |
| 5 | 1 | 1 | PMSF | 1 (mM) | |
| 6 | 1 | 1 | benzamidine | 1 (mM) | |
| 7 | 1 | 1 | ethylene glycol | 25 (%) | |
| 8 | 1 | 1 | PEG8000 | 12 (%) |
