1A0O
CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY
Experimental procedure
Source type | SYNCHROTRON |
Source details | LURE BEAMLINE DW32 |
Synchrotron site | LURE |
Beamline | DW32 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-03 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.900, 156.970, 65.970 |
Unit cell angles | 90.00, 91.70, 90.00 |
Refinement procedure
Resolution | 27.000 - 2.950 |
R-factor | 0.187 |
Rwork | 0.187 |
R-free | 0.23500 |
Structure solution method | MIRAS, DENSITY MODIFICATION, MOLECULAR REPLACEMENT |
Starting model (for MR) | 1chn |
RMSD bond length | 0.007 |
RMSD bond angle | 22.800 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | SHARP |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 3.030 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.054 | 0.099 |
Number of reflections | 41612 | |
<I/σ(I)> | 9.5 | 6.8 |
Completeness [%] | 92.0 | 86.4 |
Redundancy | 2.5 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.9 * | 20 * | drop was mixed with an equal volume of reservoir * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 25 (mM) | |
3 | 1 | reservoir | MES | 100 (mM) | |
4 | 1 | reservoir | malonic acid | 100 (mM) | |
5 | 1 | reservoir | dithiothreitol | 20 (mM) | |
6 | 1 | reservoir | 10 (mM) | ||
7 | 1 | reservoir | 10 (mM) |