1A0K
PROFILIN I FROM ARABIDOPSIS THALIANA
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09 |
Detector | RIGAKU |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.050, 43.530, 59.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.200 |
R-factor | 0.172 |
Rwork | 0.172 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nul |
RMSD bond length | 0.014 |
RMSD bond angle | 23.650 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.0) |
Refinement software | X-PLOR (3.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.066 | 0.120 |
Total number of observations | 48373 * | |
Number of reflections | 5530 | |
<I/σ(I)> | 0.2176 | 10.13 |
Completeness [%] | 90.4 | 55.5 |
Redundancy | 2.6 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5 | 2.0 M AMMONIUM SULFATE, 0.1 M CITRATE PH 5.0, 10 MM DTT, 0.2 MM EDTA |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
2 | 1 | reservoir | citrate | 0.1 (M) | |
3 | 1 | reservoir | DTT | 10 (mM) | |
4 | 1 | reservoir | EDTA | 0.2 (mM) |