1A0J
CRYSTAL STRUCTURE OF A NON-PSYCHROPHILIC TRYPSIN FROM A COLD-ADAPTED FISH SPECIES.
Experimental procedure
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM1A |
Synchrotron site | ESRF |
Beamline | BM1A |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1996-08 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.907, 83.107, 154.787 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.700 |
R-factor | 0.173 |
Rwork | 0.173 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3PTB BOVINE TRYPSIN WITH CORRECT AMINO ACID SEQUENCE SUPERIMPOSED ONTO ANIONIC SALMON TRYPSIN 2TBS. |
RMSD bond length | 0.013 |
RMSD bond angle | 26.350 * |
Data reduction software | DENZO |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.000 | 1.790 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.123 | 0.258 |
Total number of observations | 451079 * | |
Number of reflections | 76357 | |
<I/σ(I)> | 3.6 | 2.9 |
Completeness [%] | 81.2 | 52.3 |
Redundancy | 5.9 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 4.6 | 310 * | drop contained 1:1 mixture of protein and reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | benzamidine | 60 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 1.60-1.75 (M) | |
4 | 1 | reservoir | acetate | 0.1 (M) |