1A0J
CRYSTAL STRUCTURE OF A NON-PSYCHROPHILIC TRYPSIN FROM A COLD-ADAPTED FISH SPECIES.
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM1A |
| Synchrotron site | ESRF |
| Beamline | BM1A |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.907, 83.107, 154.787 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3PTB BOVINE TRYPSIN WITH CORRECT AMINO ACID SEQUENCE SUPERIMPOSED ONTO ANIONIC SALMON TRYPSIN 2TBS. |
| RMSD bond length | 0.013 |
| RMSD bond angle | 26.350 * |
| Data reduction software | DENZO |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.123 | 0.258 |
| Total number of observations | 451079 * | |
| Number of reflections | 76357 | |
| <I/σ(I)> | 3.6 | 2.9 |
| Completeness [%] | 81.2 | 52.3 |
| Redundancy | 5.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 4.6 | 310 * | drop contained 1:1 mixture of protein and reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | benzamidine | 60 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 1.60-1.75 (M) | |
| 4 | 1 | reservoir | acetate | 0.1 (M) |
