1A0B
HISTIDINE-CONTAINING PHOSPHOTRANSFER DOMAIN OF ARCB FROM ESCHERICHIA COLI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.560, 34.930, 110.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.060 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.27100 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 19.400 * |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | XTALVIEW |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.250 | |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.045 | 0.110 |
| Total number of observations | 25850 * | |
| Number of reflections | 6999 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 87.0 | 83 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.1 | 277 * | Kato, M., (1996) Acta Crystallogr.,Sect.D, 52, 1214. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG550 | 13 (%) | |
| 2 | 1 | drop | protein | 10.6 (mg/ml) | |
| 3 | 1 | drop | sodium acetate | 50 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | PEG550 | 12.5 (%) |
