1A0A

PHOSPHATE SYSTEM POSITIVE REGULATORY PROTEIN PHO4/DNA COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	288
Detector technology	DIFFRACTOMETER
Collection date	1996-06
Detector	WEISSENBERG
Spacegroup name	P 21 21 21
Unit cell lengths	53.510, 68.300, 108.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.23 *
Rwork	0.230
R-free	0.28400
Structure solution method	MIR
RMSD bond length	0.011
RMSD bond angle	23.900 *
Data reduction software	WEIS
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.087	0.202
Total number of observations	36943 *
Number of reflections	9398
<I/σ(I)>	4.2	3.5
Completeness [%]	92.0	76.8 *
Redundancy	3.9	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	3.6		VAPOR DIFFUSION METHOD: DROP-0.4MM PROTEIN, 0.2MM DNA, 1% PEG6K, 20MM NACITRATE (PH3.6), RESERVOIR-1% PEG6K, 20MM NACITRATE(PH3.6), vapor diffusion

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 6000
2	1	1	NA CITRATE
3	1	2	PEG 6000
4	1	2	NA CITRATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.4 (mM)
2	1	drop	DNA	0.2 (mM)
3	1	drop	PEG6000	1 (%(w/v))
4	1	drop	sodium citrate	20 (mM)
5	1	reservoir	PEG6000	1 (%(w/v))
6	1	reservoir	sodium citrate	20 (mM)