1A07
C-SRC (SH2 DOMAIN) COMPLEXED WITH ACE-MALONYL TYR-GLU-(N,N-DIPENTYL AMINE)
Experimental procedure
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1993-11 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.500, 67.200, 74.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.200 |
R-factor | 0.192 |
Rwork | 0.192 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1shd |
RMSD bond length | 0.015 |
RMSD bond angle | 23.090 * |
Data reduction software | R-AXIS |
Data scaling software | R-AXIS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.000 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.093 | 0.120 |
Number of reflections | 12012 | |
<I/σ(I)> | 17 | 5.3 |
Completeness [%] | 86.2 | 83 |
Redundancy | 3.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 4 * | PROTEIN WAS CRYSTALLIZED FROM 0.1 M MES, PH 6.5, 0.05 M CSCL, 30% JEFFAMINE M-600 AT 4 DEGREES C., pH 8.0, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 125 (mg/ml) | |
2 | 1 | drop | HEPES | 20 (mM) | |
3 | 1 | drop | 350 (mM) | ||
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | drop | EDTA | 5 (mM) | |
6 | 1 | reservoir | ammonium sulfate | 2 (M) |