1A06
CALMODULIN-DEPENDENT PROTEIN KINASE FROM RAT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1995-09 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.200, 76.000, 148.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.31300 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.320 |
| Total number of observations | 75340 * | |
| Number of reflections | 12191 | |
| <I/σ(I)> | 20 | 10 |
| Completeness [%] | 95.0 | 89.2 * |
| Redundancy | 4 | 74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 21 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium salfate | 2 (M) | |
| 2 | 1 | reservoir | PEG400 | 2 (%(w/v)) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | |
| 4 | 1 | drop | 70 (mM) |
