1A06

CALMODULIN-DEPENDENT PROTEIN KINASE FROM RAT

Experimental procedure

Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1995-09
Spacegroup name	I 2 2 2
Unit cell lengths	65.200, 76.000, 148.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.201
Rwork	0.201
R-free	0.31300
Structure solution method	SIRAS
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.060	0.320
Total number of observations	75340 *
Number of reflections	12191
<I/σ(I)>	20	10
Completeness [%]	95.0	89.2 *
Redundancy	4	74

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	21 *	used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium salfate	2 (M)
2	1	reservoir	PEG400	2 (%(w/v))
3	1	reservoir	HEPES	100 (mM)
4	1	drop		70 (mM)