1A05
CRYSTAL STRUCTURE OF THE COMPLEX OF 3-ISOPROPYLMALATE DEHYDROGENASE FROM THIOBACILLUS FERROOXIDANS WITH 3-ISOPROPYLMALATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-02 |
| Detector | RIGAKU |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.540, 114.240, 130.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.1977 |
| Rwork | 0.198 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ipd |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.700 * |
| Data reduction software | PROCESS |
| Data scaling software | PROCESS |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.250 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.142 |
| Number of reflections | 44212 | |
| <I/σ(I)> | 11 | 3.5 |
| Completeness [%] | 92.0 | 86 |
| Redundancy | 3.7 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 16 * | pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 10-20 (%) | |
| 3 | 1 | reservoir | MES | 0.1 (M) | |
| 4 | 1 | reservoir | IPM | 20 (mM) | |
| 5 | 1 | reservoir | 100 (mM) |
